BISAC NAT010000 Ecology
BISAC NAT045050 Ecosystems & Habitats / Coastal Regions & Shorelines
BISAC NAT025000 Ecosystems & Habitats / Oceans & Seas
BISAC NAT045030 Ecosystems & Habitats / Polar Regions
BISAC SCI081000 Earth Sciences / Hydrology
BISAC SCI092000 Global Warming & Climate Change
BISAC SCI020000 Life Sciences / Ecology
BISAC SCI039000 Life Sciences / Marine Biology
BISAC SOC053000 Regional Studies
BISAC TEC060000 Marine & Naval
Nutrient dynamics in seagrass beds and nutrient demands of seagrass biomass are not clear, although nutrient uptake of seagrass has been experimentally studied in the laboratory. We conducted the field observations and the bottom sediment core incubations to estimate nutrient fluxes in the seagrass, Zostera marina meadow. DIN (nitrate, nitrite and ammonium) concentrations were always low particularly during the Z. marina growing season (from spring to summer), and water exchanges caused by tidal currents hardly supplied nutrient demand for Z. marina. Sediment pore water also supplied insufficient nutrients to Z. marina, because pore water had less volume than the water column, although DIN concentrations of pore water were 10-100 fold higher than those of the water column. Nutrient flux from sediment to water column estimated by the sediment core incubation experiments showed a similar rate with tidal water exchange. Thus, our results suggested that Z. marina adapted for low nutrient concentrations and each nutrient source in the Z. marina meadow slightly contributed but could not support Z. marina growth. We found that another nutrient source, for example, precipitation, supplied high DIN to the Z. marina meadow. After rainfall, the DIN concentration of seawater in the Z. marina meadow increased 2-5 times higher. Moreover, nitrogen content of eelgrass also increased 2-3 times higher during several days. Those results suggested that Z. marina was usually exposed to a low nutrient concentration but could uptake abundant nutrients from temporary nutrient supplies such as precipitation.
Zostera marina, nutrient, seagrass meadow
It is well known that eelgrass (Zostera marina) meadows have a great role in the coastal ecosystem but are often degraded due to artificial eutrophication. In Japan, in spite of much efforts to revive Z. marina meadows, they hardly worked due to the lack of information about Z. marina nutrient demand and utilization. Most studies according to Z. marina nutrient utilization were conducted by laboratory experiments. It is very important to understand the nutrient dynamics and the nutrient demand in Z. marina meadows.
We investigated the nutrient dynamics in a small inlet (420,000 m2), Ikushima Bay, where Z. marina covered almost the whole area and had no inflow river. Ikushima Bay is connected to Bisan-seto, located in the eastern part of the Seto Inland Sea, Japan (Fig. 1). Bisan-seto is one of the largest Z. marina habitats in Japan. The water movement of Ikushima Bay is simply regulated by tidal current. Since it involves simple water movement, it is easy to know the nitrogen budget in Ikushima Bay. Our objective was to confirm the nutrient dynamics in the Z. marina meadows.
II. Nitrogen budget
We measured nutrient fluxes between offshore water and Ikushima Bay. In spring, the Z. marina growing season, dissolved inorganic nitrogen (DIN) inflow from offshore water was estimated to be 8 mg N m-2 day-1. We also measured nutrient flux from sediment to the water column by a core incubation method. DIN inflow from sediment was estimated at 7 mg N m-2 day-1. Our results indicated that the Z. marina meadow in Ikushima Bay was functioning as a DIN sink.
Then, we estimated nitrogen demand of Z. marina by its growth rate, nitrogen content and standing stock. The nitrogen demand of Z. marina in spring was estimated at 160 mg N m-2 day-1 (Fig. 2). Even if our estimation of nitrogen demand of Z. marina was overestimated, the nitrogen demand of Z. marina (160 mg N m-2 day-1) was significantly higher than the DIN influx into Ikushima Bay. This result indicated that DIN inflow to the Z. marina meadow was not balanced with the nitrogen demand of Z. marina, and there were other nitrogen sources in sediment or temporary nitrogen discharges to Ikushima Bay.
III. Pore water nutrient
Another nitrogen source, underground nitrogen pools, which could be uptaken from the root of Z. marina, may be important . We measured the annual sediment pore water DIN concentrations. During spring and summer, when Z. marina was growing, the mean pore water DIN concentration was 50 µM. Then, the pore water DIN concentration increased with the standing stock of Z. marina decreasing in autumn. During winter, when almost all Z. marina disappeared, the pore water DIN concentration was increased by 67 µM. DIN decrease of pore water during spring and summer might be due to the uptake by Z. marina. However, water volume of the pore water was 4-5 digit lower than that of the water column. Although the pore water DIN concentrations were higher than those of the water column, we concluded that pore water DIN scarcely contributed to Z. marina growth.
IV. Nitrogen uptake of eelgrass
Seagrasses show high nutrient uptake affinities , and Z. marina could utilize intermittent nutrient supply such as rainfall and freshwater discharge. We measured the variation of nitrogen content of Z. marina in the short term such as a few days. After rainfall, the nitrogen content of Z. marina increased from 14 to 35 mg N g dw-1 with the increasing water column DIN concentration over a few days. This result suggested that Z. marina could immediately uptake DIN for intermittent nitrogen supply.
It was revealed that each nitrogen source could not sustain the Z. marina nitrogen demand alone in Ikushima Bay, although there were various nitrogen pools and discharges into the bay. Our results suggested that Z. marina utilized multiple nitrogen sources so as to conform to the situation.
This research was partly supported by the Environment Research and Technology Development Fund (S-13) of the Ministry of the Environment, Japan.
1. F.T. Short, “The seagrass, Zostera marina L. plant morphology and bed structure in relation to sediment ammonium in Izembek Lagoon, Alaska,” Aquatic Botany, vol. 16, pp. 149-161, 1983
2. B.W. Touchette, and J.M. Burkholder, “Review of nitrogen and phosphorus metabolism in seagrasses,” Journal of Experimental Marine Biology and Ecology, vol. 250, pp. 133-167, 2000